https://dspace.nii.res.in//https://dspace.nii.res.in/handle/123456789/23 Former Principal Investigator- Dr. Lalit C. Garg, Present Principal Investigator- Dr. Anil Kumar 2026-04-07T17:28:57Z https://dspace.nii.res.in//https://dspace.nii.res.in/handle/123456789/1630 Title: MIP based sensor for detection of gut microbiota-derived Indoxyl Sulfate using PANI- Graphene-NiS2. Authors: Dalal, N; Dhiman, TK; Lakshmi, GBVS; Singh, AK; Solanki, PR; Kumar, A Abstract: Indoxyl sulphate (IS) is a gut microbiota-derived metabolite that is involved in human health and dis- eases. The normal range of IS in human serum may be in the range of 0.024e0.05 mg/dl, but the altered IS level has been observed in diseased conditions, such as chronic kidney diseases, diabetes and cancer, which implicate IS as a diagnostic and prognostic biomarker. In the current work, a molecularly imprinted polymer (MIP)-based electrochemical sensor was made for the quantification of IS in a wide concentration range. The IS sensing was studied using DPV, revealing decreasing peak current with increasing IS concentrations. The MIP/ITO electrode has shown a good response from 1.0 pM to 6.0 mM which is linear in trend, with a sensitivity of 0.0362 mA (log (ng/mL)) -1 cm -2 and a lower limit of detection of 0.286 pg/mL and a low response time of 5 min. The interference study also confirmed the specificity of MIP-based electrochemical sensor toward IS detection. The real sample study confirmed the MIP-based sensor's applicability to detect IS in urine samples. The sensitive, cheap, and reproducible MIP-based detection of IS may offer a new diagnostic approach to diagnose various diseases implicated with altered level of IS in human serum and urine. 2022-01-01T00:00:00Z https://dspace.nii.res.in//https://dspace.nii.res.in/handle/123456789/1586 Title: N-acetyl-L-tryptophan provides radioprotection to mouse and primate models by antagonizing the TRPV1 receptor and substance P inhibition. Authors: Kumar, Raj; Kumari, Pratibha; Gaurav, Neelanshu; Kumar, Ravi; Singh, Darshana; Malhotra, Poonam; Singh, Shravan Kumar; Bhatta, Rabi Sankar; Kumar, Anil; Nagarajan, Perumal; Singh, Surender; Dalal, Nishu; Roy, Bal Gangadhar; Bhatt, Anant Narayan; Chandna, Sudhir Abstract: Purpose: The present study was carried out to evaluate the radioprotective activities of N-acetyl-L-tryptophan (L-NAT) using rodent and non-human primate (NHP) models. Materials and methods: The antagonistic effect of L-NAT on the Transient receptor potential vanilloid-1 (TRPV1) receptor and substance P inhibition was determined using molecular docking and Elisa assays. The in vivo radioprotective activity of L-NAT was evaluated using whole-body survival assays in mice and NHPs. Radioprotective activity of L-NAT was also determined at the systemic level using quantitative histological analysis of bone marrow, jejunum, and seminiferous tubules of irradiated mice. Results: Molecular docking studies revealed a strong binding of L-NAT with TRPV1 receptor at similar binding pockets to which capsaicin, an agonist of the TRPV1 receptor, binds. Further, capsaicin and gamma radiation were found to induce substance P levels in the intestines and serum of the mice, while L-NAT pretreatment was found to inhibit it. Significant whole-body survival (>80%) was observed in irradiated (9.0 Gy) mice that pretreated with L-NAT (150 mg/kg, b.wt. im) compared to 0% survival in irradiated mice that not pretreated with L-NAT. The quantitative histology of the hematopoietic, gastrointestinal, and male reproductive systems demonstrated significant protection against radiation-induced cellular degeneration. Interestingly, 100% survival was observed with irradiated NHPs (6.5 Gy) that pretreated with L-NAT (37.5 mg/kg, b.wt.im). Significant improvement in the hematology profile was observed after days 10-20 post-treatment periods in irradiated (6.5 Gy) NHPs that were pretreated with L-NAT. Conclusion: L-NAT demonstrated excellent radioprotective activity in the mice and NHP models, probably by antagonizing TRPV1 receptor and subsequently inhibiting substance P expression. 2025-01-01T00:00:00Z https://dspace.nii.res.in//https://dspace.nii.res.in/handle/123456789/1585 Title: Interaction studies unveil potential binding sites on bovine serum albumin for gut metabolite trimethylamine n-oxide (TMAO) Authors: Kumar, Anil; Gulati, Payal; Lakshmi, Gbvs; Mohan, Anand; Sharma, Geeta Raj; Solanki, Pratima R; Verma, Awadhesh Kumar Abstract: Trimethylamine-N-oxide (TMAO) is gut microbiota-derived metabolite, plays a critical role in human health and diseases such as metabolic, cardiovascular, colorectal cancer and, neurological disorders. Binding interactions between TMAO and serum albumins are crucial to understand the impact of TMAO on disease mechanisms. However, detailed insights into the interaction mechanisms, preferred binding locations, and conformational changes in BSA upon binding TMAO are still unclear. TMAO interacts with serum albumin in human body and thus, a model study of interaction for TMAO-BSA conjugate is presented in support of it. Decrease in absorbance intensity of protein upon interaction with metabolites reveals conjugate formation, while fluorescence spectroscopy indicate static quenching. Contact angle measurements further reveal the hydrophilic nature of the TMAO-BSA complex, while CD and FTIR support conformational changes in BSA upon binding but structure remain intact. Computational studies, such as molecular docking, molecular dynamics simulation and, MM/GBSA, confirm a stable complex with a binding energy of - 3.6 kcal/mol. These findings provide a foundation for understanding the pharmacodynamics and pharmacokinetics of TMAO and may aid in developing strategies for treating diseases, such as chronic kidney disease and neurological disorder where TMAO-serum albumins interaction are implicated. 2025-01-01T00:00:00Z https://dspace.nii.res.in//https://dspace.nii.res.in/handle/123456789/1582 Title: Optical tuning of polymer functionalized zinc oxide quantum dots as a selective probe for the detection of antibiotics Authors: Verma, Awadhesh Kumar; Lakshmi, G B V S; Dhiman, Tarun Kumar; Hashmi, S Z H; Kumar, Anil; Solank, Pratima R Abstract: Excess consumption of antibiotics leads to antibiotic resistance that hinders the control and cure of microbial diseases. Therefore, it is crucial to monitor the antibiotic levels in the environment. In this proposed research work, an optical nano-sensor was devised that can sense the ultra-low concentration of antibiotics, in samples like tap water using fluorescent zinc oxide quantum dots (ZnO QDs) based nano-sensor. For this, different polymers (polyvinylalcohol-PVA and polyvinylpyrrolidine-PVP) capped florescent ZnO QDs were synthesized using a modified sol-gel technique. These were used as fluorescent probes to monitor the presence of antibiotics. The optical characterizations of synthesized QDs were performed using UV-visible absorption and fluorescence spectroscopic methods while structural characteristics were analyzed by using Raman spectroscopy and X-ray diffraction spectroscopy. The formation of capped QDs was confirmed by Fourier transform infrared spectroscopy (FTIR). Charge on the synthesized QDs was obtained with the help of ZETA potential. Here ten different antibiotics were checked, Ciprofloxacin and Moxifloxacin have shown excellent sensing and specificity with PVA-ZnO QDs and PVP-ZnO QDs with LOD of 1.4 nM and 0.8 nM, and sensitivity of 36.17 units/mM and 19.33 units/mM respectively. This study also inferred the tuning of the ZnO QDs properties and specificity towards the different antibiotics can be achieved by capping QDs with different polymers. 2025-01-01T00:00:00Z