https://dspace.nii.res.in//https://dspace.nii.res.in/handle/123456789/128 2026-04-27T15:59:14Z 2026-04-27T15:59:14Z Sharma, Naveen Akhade, Ajay Suresh Ismaeel, Sana Qadri, Ayub https://dspace.nii.res.in//https://dspace.nii.res.in/handle/123456789/1525 2025-12-05T12:47:36Z 2021-01-01T00:00:00Z

Title: Serum-borne lipids amplify TLR-activated inflammatory responses Authors: Sharma, Naveen; Akhade, Ajay Suresh; Ismaeel, Sana; Qadri, Ayub Abstract: TLRs recognize conserved pathogen associated molecular patterns and generate innate immune responses. Several circulating and cell membrane associated proteins have been shown to collaborate with TLRs in sensing microbial ligands and promoting inflammatory responses. Here, we show that serum and serum-borne lipids including lysophosphatidylcholine (LPC) amplify inflammatory responses from intestinal epithelial cells and mononuclear phagocytes primed with microbial TLR ligands. Treatment with the inhibitors of G protein-coupled receptor (GPCR) signaling, suramin, or pertussis toxin (PT), the inhibitor of JNK-MAPK, or knockdown of LPC response-regulating GPCR, G2A, decreases the augmentation brought about by serum or LPC in TLR-induced inflammatory response. In vivo administration of PT or anti-G2A antibody reduces TLR2-activated cytokine secretion. The ability of host lipids to costimulate TLR-generated cellular responses represents a novel pathway for the amplification of innate immunity and inflammation.

2021-01-01T00:00:00Z Mohan, K. Varsha Sinha, Prakriti Swami, Bharati Muniyasamy, Abaranjitha Nagarajan1, Perumal Upadhyay, Pramod https://dspace.nii.res.in//https://dspace.nii.res.in/handle/123456789/1474 2025-12-05T12:47:57Z 2021-01-01T00:00:00Z

Title: Microdissection of the Rodent Eye Authors: Mohan, K. Varsha; Sinha, Prakriti; Swami, Bharati; Muniyasamy, Abaranjitha; Nagarajan1, Perumal; Upadhyay, Pramod Abstract: The ocular micro-dissection of the rodent eye involves the segmentation of the enucleated eyeball with the attached nictitating membrane, or third eyelid, to obtain the anterior and posterior eyecups. With this technique, the sub-parts of the eye, including the corneal tissue, neural tissue, retinal pigment epithelial (RPE) tissue, and lens, can be obtained for wholemounts, cryo-sectioning, and/or single-cell suspensions of a specific ocular tissue. The presence of the third eyelid presents unique and significant advantages, as it benefits the maintenance of the orientation of the eye, which is important for understanding eye physiology following any localized intervention or in studies involving ocular analysis relating to the eye's spatial topography. In this method, we enucleated the eyeball at the socket along with the third eyelid by carefully and slowly cutting through the extraocular muscles and severing the optic nerve. The eyeball was pierced through the corneal limbus using a microblade. The incision was used as the point of entry, allowing for cutting along the corneal-scleral junction by inserting micro-scissors through the incision point. Small and continuous cuts along the circumference were made until the cups separated. These could be further dissected by gently peeling the translucent layer of the neural retina using Colibri suturing forceps to obtain the neural retina and RPE layers. Further, three/ four equidistant cuts were made from the periphery perpendicularly to the optic center until the optic nerve was reached. This opened the hemispherical cups into a floret shape so that they fell flat and could be easily mounted. This technique has been used in our lab for corneal wholemounts and retinal sections. The presence of the third eyelid delineates the nasal-temporal orientation, which allows for the study of various cell therapy interventions post-transplantation and, thus, the targeted physiological validation vital for visualization and accurate representation in such studies.

2021-01-01T00:00:00Z Qadri, Ayub https://dspace.nii.res.in//https://dspace.nii.res.in/handle/123456789/1333 2025-12-05T12:45:05Z 2015-01-01T00:00:00Z

Title: MyD88-dependent pro-inflammatory activity in Vi polysaccharide vaccine against typhoid promotes Ab switching to IgG Authors: Qadri, Ayub Abstract: Vi capsular polysaccharide is currently in use as a vaccine against human typhoid caused by Salmonella Typhi. The vaccine efficacy correlates with IgG anti-Vi Abs. We have recently reported that Vi can generate inflammatory responses through activation of the TLR2/TLR1 complex. In the present study, we show that immunization with Vi produces IgM as well as IgG Abs in wild type mice. This ability is not compromised in mice deficient in T cells. However, immunization of mice lacking the TLR adaptor protein, MyD88, with Vi elicits only IgM Abs. These results suggest that MyD88-dependent pro-inflammatory ability of the Vi vaccine might be vital in generating IgG Abs with this T-independent Ag.

2015-01-01T00:00:00Z Qadri, Ayub Yadav, Jitender Ismaeel, Sana https://dspace.nii.res.in//https://dspace.nii.res.in/handle/123456789/1248 2025-12-05T12:41:16Z 2020-01-01T00:00:00Z

Title: Lysophosphatidylcholine Potentiates Antibacterial Activity of Polymyxin B Authors: Qadri, Ayub; Yadav, Jitender; Ismaeel, Sana Abstract: Polymyxin B, used to treat infections caused by antibiotic-resistant Gram-negative bacteria, produces nephrotoxicity at its current dosage. We show that a combination of nonbactericidal concentration of this drug and lysophosphatidylcholine (LPC) potently inhibits growth of Salmonella and at least two other Gram-negative bacteria in vitro This combination makes bacterial membrane porous and causes degradation of DnaK, the regulator of protein folding. Polymyxin B-LPC combination may be an effective and safer regimen against drug-resistant bacteria.

2020-01-01T00:00:00Z